da molecularpost | Mag 25, 2023 | Biologia Molecolare, Liver, Organoids
Genetic engineering of human fetal and adult liver organoids by CRISPR-Cas9 technology Fig. 1 – Schematic overview of this protocol (Created with BioRender.com) Abstract The liver is composed of two types of epithelial cells: hepatocytes and liver ductal cells....
da molecularpost | Mag 20, 2023 | Biologia Molecolare, Liver, Organoids
Genetic engineering of human organoids using a homology independent CRISPR-Cas9 Figure 1 – Comparison between HDR and NHEJ mechanism. In the HDR mechanism are highlighted the homology arms (HA). Created with Biorender. Abstract Organoids are in vitro generated...
da molecularpost | Giu 15, 2021 | Biologia Molecolare, Liver, Organoids
Liver organoids: characteristics and future perspectives Abstract Liver bud organoids offer an alternative to traditional 2D cell cultures, but in order to exploit all their potential it is necessary to understand how cell communication affects differentiation. Camp...